BD HorizonTM BV605 Mouse Anti-Human CD40

Description

Multiparameter flow cytometric analysis of CD40 expression on Human peripheral blood leukocyte populations. Platelet-depleted whole blood was first treated with BD FACS Lysing Solution Cat. No. 349202 to lyse erythrocytes. After washing, the leukocytes were then stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plot or BD Horizon BV605 Mouse Anti-Human CD40 antibody Cat. No. 569044/569045; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD40 expression on Human peripheral blood leukocyte populations. Platelet-depleted whole blood was first treated with BD FACS Lysing Solution Cat. No. 349202 to lyse erythrocytes. After washing, the leukocytes were then stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plot or BD Horizon BV605 Mouse Anti-Human CD40 antibody Cat. No. 569044/569045; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD40 expression on Human peripheral blood leukocyte populations. Platelet-depleted whole blood was first treated with BD FACS Lysing Solution Cat. No. 349202 to lyse erythrocytes. After washing, the leukocytes were then stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plot or BD Horizon BV605 Mouse Anti-Human CD40 antibody Cat. No. 569044/569045; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD40 expression on Human peripheral blood leukocyte populations. Platelet-depleted whole blood was first treated with BD FACS Lysing Solution Cat. No. 349202 to lyse erythrocytes. After washing, the leukocytes were then stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plot or BD Horizon BV605 Mouse Anti-Human CD40 antibody Cat. No. 569044/569045; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD40 expression on Human peripheral blood leukocyte populations. Platelet-depleted whole blood was first treated with BD FACS Lysing Solution Cat. No. 349202 to lyse erythrocytes. After washing, the leukocytes were then stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plot or BD Horizon BV605 Mouse Anti-Human CD40 antibody Cat. No. 569044/569045; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD40 expression on Human peripheral blood leukocyte populations. Platelet-depleted whole blood was first treated with BD FACS Lysing Solution Cat. No. 349202 to lyse erythrocytes. After washing, the leukocytes were then stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plot or BD Horizon BV605 Mouse Anti-Human CD40 antibody Cat. No. 569044/569045; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.