BD HorizonTM BV605 Mouse Anti-Human CD86

Description

Multiparameter flow cytometric analysis of CD86 expression on Human peripheral blood leukocyte populations. Fresh whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BV605 Mouse IgG2b, kappa Isotype Control Cat. No. 563099; Left Plot or BD Horizon BV605 Mouse Anti-Human CD86 antibody Cat. No. 569304/569305; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD86 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either BD Horizon BV605 Mouse IgG2b, kappa Isotype Control dashed line histogram or BD Horizon BV605 Mouse Anti-Human CD86 antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The fluorescence histogram showing CD86 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD86 expression on Human peripheral blood leukocyte populations. Fresh whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BV605 Mouse IgG2b, kappa Isotype Control Cat. No. 563099; Left Plot or BD Horizon BV605 Mouse Anti-Human CD86 antibody Cat. No. 569304/569305; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD86 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either BD Horizon BV605 Mouse IgG2b, kappa Isotype Control dashed line histogram or BD Horizon BV605 Mouse Anti-Human CD86 antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The fluorescence histogram showing CD86 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD86 expression on Human peripheral blood leukocyte populations. Fresh whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leukocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with either BD Horizon BV605 Mouse IgG2b, kappa Isotype Control Cat. No. 563099; Left Plot or BD Horizon BV605 Mouse Anti-Human CD86 antibody Cat. No. 569304/569305; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD86 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either BD Horizon BV605 Mouse IgG2b, kappa Isotype Control dashed line histogram or BD Horizon BV605 Mouse Anti-Human CD86 antibody solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The fluorescence histogram showing CD86 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.