
562977 ·
BDB Bioscience · Cat: 562977
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Two-color flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV605 Mouse IgG1, k Isotype Control Cat. No. 562652; Left Panel or BD Horizon BV605 Mouse Anti-Human IgM antibody Cat. No. 562977; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgM or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV605 Mouse IgG1, k Isotype Control Cat. No. 562652; Left Panel or BD Horizon BV605 Mouse Anti-Human IgM antibody Cat. No. 562977; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgM or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV605 Mouse IgG1, k Isotype Control Cat. No. 562652; Left Panel or BD Horizon BV605 Mouse Anti-Human IgM antibody Cat. No. 562977; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgM or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV605 Mouse IgG1, k Isotype Control Cat. No. 562652; Left Panel or BD Horizon BV605 Mouse Anti-Human IgM antibody Cat. No. 562977; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgM or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV605 Mouse IgG1, k Isotype Control Cat. No. 562652; Left Panel or BD Horizon BV605 Mouse Anti-Human IgM antibody Cat. No. 562977; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgM or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IgM expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and either BD Horizon BV605 Mouse IgG1, k Isotype Control Cat. No. 562652; Left Panel or BD Horizon BV605 Mouse Anti-Human IgM antibody Cat. No. 562977; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgM or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.