BD HorizonTM BV605 Mouse Anti-Human IL-2

Description

Two color flow cytometric analysis of IL-2 expression by activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 , washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 , and then stained with FITC Mouse Anti-Human CD3 antibody Cat No. 555332/561806/561807 and either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat No. 562652, Left Panel or BD Horizon BV605 Mouse Anti-Human IL-2 antibody Cat No. 563947, Right Panel by using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric dot plots show the correlated expression of IL-2 or Ig Isotype control staining versus CD3 and were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two color flow cytometric analysis of IL-2 expression by activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 , washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 , and then stained with FITC Mouse Anti-Human CD3 antibody Cat No. 555332/561806/561807 and either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat No. 562652, Left Panel or BD Horizon BV605 Mouse Anti-Human IL-2 antibody Cat No. 563947, Right Panel by using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric dot plots show the correlated expression of IL-2 or Ig Isotype control staining versus CD3 and were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two color flow cytometric analysis of IL-2 expression by activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 , washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 , and then stained with FITC Mouse Anti-Human CD3 antibody Cat No. 555332/561806/561807 and either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat No. 562652, Left Panel or BD Horizon BV605 Mouse Anti-Human IL-2 antibody Cat No. 563947, Right Panel by using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric dot plots show the correlated expression of IL-2 or Ig Isotype control staining versus CD3 and were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two color flow cytometric analysis of IL-2 expression by activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 , washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 , and then stained with FITC Mouse Anti-Human CD3 antibody Cat No. 555332/561806/561807 and either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat No. 562652, Left Panel or BD Horizon BV605 Mouse Anti-Human IL-2 antibody Cat No. 563947, Right Panel by using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric dot plots show the correlated expression of IL-2 or Ig Isotype control staining versus CD3 and were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two color flow cytometric analysis of IL-2 expression by activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 , washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 , and then stained with FITC Mouse Anti-Human CD3 antibody Cat No. 555332/561806/561807 and either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat No. 562652, Left Panel or BD Horizon BV605 Mouse Anti-Human IL-2 antibody Cat No. 563947, Right Panel by using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric dot plots show the correlated expression of IL-2 or Ig Isotype control staining versus CD3 and were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two color flow cytometric analysis of IL-2 expression by activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 , washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 , and then stained with FITC Mouse Anti-Human CD3 antibody Cat No. 555332/561806/561807 and either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat No. 562652, Left Panel or BD Horizon BV605 Mouse Anti-Human IL-2 antibody Cat No. 563947, Right Panel by using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric dot plots show the correlated expression of IL-2 or Ig Isotype control staining versus CD3 and were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.