BD HorizonTM BV605 Mouse Anti-Human TSLP Receptor

Description

Flow cytometric analysis of human TSLP Receptor expression on human TSLPR-transfected cells. HEK-293 cells cotransfected with constructs coding for human TSLPR and IL-7Ralpha/CD127 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then frozen and stored at -80C for subsequent testing. The frozen cells were thawed, their freezing media was removed, and the cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . The cells were then stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; dashed line histogram or BD Horizon BV605 Mouse Anti-Human TSLPR antibody Cat. No. 563341; solid line histogram . The flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of human TSLP Receptor expression on human TSLPR-transfected cells. HEK-293 cells cotransfected with constructs coding for human TSLPR and IL-7Ralpha/CD127 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then frozen and stored at -80C for subsequent testing. The frozen cells were thawed, their freezing media was removed, and the cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . The cells were then stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; dashed line histogram or BD Horizon BV605 Mouse Anti-Human TSLPR antibody Cat. No. 563341; solid line histogram . The flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of human TSLP Receptor expression on human TSLPR-transfected cells. HEK-293 cells cotransfected with constructs coding for human TSLPR and IL-7Ralpha/CD127 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then frozen and stored at -80C for subsequent testing. The frozen cells were thawed, their freezing media was removed, and the cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 . The cells were then stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; dashed line histogram or BD Horizon BV605 Mouse Anti-Human TSLPR antibody Cat. No. 563341; solid line histogram . The flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System.