BD HorizonTM BV605 Mouse Anti-Ki-67

Description

Two-color flow cytometric analysis of Ki-67 expression by noncycling Human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling Human peripheral blood mononuclear cells PBMC; Top Plots or proliferating cells from the Human MOLT-4 ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plots or BD Horizon BV605 Mouse Anti-Ki-67 antibody Cat. No. 567122; Right Plots and counterstained with BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of 7-AAD staining versus Ki-67 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact PBMC or MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Two-color flow cytometric analysis of Ki-67 expression by noncycling Human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling Human peripheral blood mononuclear cells PBMC; Top Plots or proliferating cells from the Human MOLT-4 ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plots or BD Horizon BV605 Mouse Anti-Ki-67 antibody Cat. No. 567122; Right Plots and counterstained with BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of 7-AAD staining versus Ki-67 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact PBMC or MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Show More Two-color flow cytometric analysis of Ki-67 expression by noncycling Human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling Human peripheral blood mononuclear cells PBMC; Top Plots or proliferating cells from the Human MOLT-4 ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon BV605 Mouse IgG1, kappa Isotype Control Cat. No. 562652; Left Plots or BD Horizon BV605 Mouse Anti-Ki-67 antibody Cat. No. 567122; Right Plots and counterstained with BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of 7-AAD staining versus Ki-67 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact PBMC or MOLT-4 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software.