
563053 ·
BDB Bioscience · Cat: 563053
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD45 expressed on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV605 Rat IgG2b, kappa Isotype Control Cat. No. 563145; dashed line histogram or a BD Horizon BV605 Rat Anti-Mouse CD45 antibody Cat.No. 563053; solid line histogram . Flow cytometric fluorescence histograms were derived from gated events based on forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD45 expressed on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV605 Rat IgG2b, kappa Isotype Control Cat. No. 563145; dashed line histogram or a BD Horizon BV605 Rat Anti-Mouse CD45 antibody Cat.No. 563053; solid line histogram . Flow cytometric fluorescence histograms were derived from gated events based on forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD45 expressed on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV605 Rat IgG2b, kappa Isotype Control Cat. No. 563145; dashed line histogram or a BD Horizon BV605 Rat Anti-Mouse CD45 antibody Cat.No. 563053; solid line histogram . Flow cytometric fluorescence histograms were derived from gated events based on forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.