BD HorizonTM BV605 Rat Anti-Mouse F4/80

Description

Multicolor flow cytometric analyses of F4/80 expression. The bivariate pseudocolor density plots showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b Mouse Splenocytes; Top Plots or CD117 c-Kit Mouse Peritoneal Exudate Cells; Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Top Plots - Mouse splenocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti- CD11b antibody Cat. No. 553311/557397 and with either BD Horizon BV605 Rat IgG2a, kappa Isotype Control Cat. No. 563144; Left Plot or BD Horizon BV605 Rat Anti-Mouse F4/80 antibody Cat. No. 569237; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bottom Plots - Mouse peritoneal exudate cells PEC . C57BL/6 Mouse PEC were similarly pretreated with Mouse BD Fc Block, stained with BD Horizon BV421 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 567818 and with either BD Horizon BV605 Rat IgG2a, kappa Isotype Control Left Plot or BD Horizon BV605 Rat Anti-Mouse F4/80 antibody Right Plot at 1 mug/test followed by DAPI Solution and analyzed. Multicolor flow cytometric analyses of F4/80 expression. The bivariate pseudocolor density plots showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b Mouse Splenocytes; Top Plots or CD117 c-Kit Mouse Peritoneal Exudate Cells; Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Top Plots - Mouse splenocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti- CD11b antibody Cat. No. 553311/557397 and with either BD Horizon BV605 Rat IgG2a, kappa Isotype Control Cat. No. 563144; Left Plot or BD Horizon BV605 Rat Anti-Mouse F4/80 antibody Cat. No. 569237; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bottom Plots - Mouse peritoneal exudate cells PEC . C57BL/6 Mouse PEC were similarly pretreated with Mouse BD Fc Block, stained with BD Horizon BV421 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 567818 and with either BD Horizon BV605 Rat IgG2a, kappa Isotype Control Left Plot or BD Horizon BV605 Rat Anti-Mouse F4/80 antibody Right Plot at 1 mug/test followed by DAPI Solution and analyzed. Show More Multicolor flow cytometric analyses of F4/80 expression. The bivariate pseudocolor density plots showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b Mouse Splenocytes; Top Plots or CD117 c-Kit Mouse Peritoneal Exudate Cells; Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Top Plots - Mouse splenocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti- CD11b antibody Cat. No. 553311/557397 and with either BD Horizon BV605 Rat IgG2a, kappa Isotype Control Cat. No. 563144; Left Plot or BD Horizon BV605 Rat Anti-Mouse F4/80 antibody Cat. No. 569237; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Bottom Plots - Mouse peritoneal exudate cells PEC . C57BL/6 Mouse PEC were similarly pretreated with Mouse BD Fc Block, stained with BD Horizon BV421 Rat Anti-Mouse CD117 c-Kit antibody Cat. No. 567818 and with either BD Horizon BV605 Rat IgG2a, kappa Isotype Control Left Plot or BD Horizon BV605 Rat Anti-Mouse F4/80 antibody Right Plot at 1 mug/test followed by DAPI Solution and analyzed.