BD HorizonTM BV605 Rat Anti-Mouse I-A/I-E

Description

Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on C57BL/6 mouse splenocytes. Splenic leucocytes from either M5/114-negative SJL Left Panel or M5/114-positive C57BL/6 Right Panel mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV605 Rat Anti-Mouse I-A/I-E Cat. No. 563413 and PE Hamster Anti-Mouse CD3 Cat. No. 553064/553063/561824 antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on C57BL/6 mouse splenocytes. Splenic leucocytes from either M5/114-negative SJL Left Panel or M5/114-positive C57BL/6 Right Panel mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV605 Rat Anti-Mouse I-A/I-E Cat. No. 563413 and PE Hamster Anti-Mouse CD3 Cat. No. 553064/553063/561824 antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on C57BL/6 mouse splenocytes. Splenic leucocytes from either M5/114-negative SJL Left Panel or M5/114-positive C57BL/6 Right Panel mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV605 Rat Anti-Mouse I-A/I-E Cat. No. 563413 and PE Hamster Anti-Mouse CD3 Cat. No. 553064/553063/561824 antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on C57BL/6 mouse splenocytes. Splenic leucocytes from either M5/114-negative SJL Left Panel or M5/114-positive C57BL/6 Right Panel mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV605 Rat Anti-Mouse I-A/I-E Cat. No. 563413 and PE Hamster Anti-Mouse CD3 Cat. No. 553064/553063/561824 antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on C57BL/6 mouse splenocytes. Splenic leucocytes from either M5/114-negative SJL Left Panel or M5/114-positive C57BL/6 Right Panel mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV605 Rat Anti-Mouse I-A/I-E Cat. No. 563413 and PE Hamster Anti-Mouse CD3 Cat. No. 553064/553063/561824 antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of I-A/I-E MHC class II alloantigen expression on C57BL/6 mouse splenocytes. Splenic leucocytes from either M5/114-negative SJL Left Panel or M5/114-positive C57BL/6 Right Panel mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV605 Rat Anti-Mouse I-A/I-E Cat. No. 563413 and PE Hamster Anti-Mouse CD3 Cat. No. 553064/553063/561824 antibodies. Two-color flow cytometric dot plots showing the expression of I-A/I-E MHC class II alloantigens versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.