BD HorizonTM BV605 Rat Anti-Mouse IL-2

Description

Two color flow cytometric analysis of IL-2 expression by stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553046/553047/561835 and either BD Horizon BV605 Rat IgG2b, kappa Isotype Control Cat. No. 563145; Left Panel or BD Horizon BV605 Rat Anti-Mouse IL-2 antibody Cat. No. 563911; Right Panel by using BD Biosciences Intracellular Cytokine Staining protocol. Flow cytometric dot plots showing correlated expression of IL-2 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two color flow cytometric analysis of IL-2 expression by stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553046/553047/561835 and either BD Horizon BV605 Rat IgG2b, kappa Isotype Control Cat. No. 563145; Left Panel or BD Horizon BV605 Rat Anti-Mouse IL-2 antibody Cat. No. 563911; Right Panel by using BD Biosciences Intracellular Cytokine Staining protocol. Flow cytometric dot plots showing correlated expression of IL-2 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two color flow cytometric analysis of IL-2 expression by stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553046/553047/561835 and either BD Horizon BV605 Rat IgG2b, kappa Isotype Control Cat. No. 563145; Left Panel or BD Horizon BV605 Rat Anti-Mouse IL-2 antibody Cat. No. 563911; Right Panel by using BD Biosciences Intracellular Cytokine Staining protocol. Flow cytometric dot plots showing correlated expression of IL-2 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two color flow cytometric analysis of IL-2 expression by stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553046/553047/561835 and either BD Horizon BV605 Rat IgG2b, kappa Isotype Control Cat. No. 563145; Left Panel or BD Horizon BV605 Rat Anti-Mouse IL-2 antibody Cat. No. 563911; Right Panel by using BD Biosciences Intracellular Cytokine Staining protocol. Flow cytometric dot plots showing correlated expression of IL-2 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two color flow cytometric analysis of IL-2 expression by stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553046/553047/561835 and either BD Horizon BV605 Rat IgG2b, kappa Isotype Control Cat. No. 563145; Left Panel or BD Horizon BV605 Rat Anti-Mouse IL-2 antibody Cat. No. 563911; Right Panel by using BD Biosciences Intracellular Cytokine Staining protocol. Flow cytometric dot plots showing correlated expression of IL-2 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two color flow cytometric analysis of IL-2 expression by stimulated mouse splenocytes. Mouse splenic leucocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested and washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553046/553047/561835 and either BD Horizon BV605 Rat IgG2b, kappa Isotype Control Cat. No. 563145; Left Panel or BD Horizon BV605 Rat Anti-Mouse IL-2 antibody Cat. No. 563911; Right Panel by using BD Biosciences Intracellular Cytokine Staining protocol. Flow cytometric dot plots showing correlated expression of IL-2 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.