BD HorizonTM BV605 Rat Anti-Mouse IL-4

Description

Two-color flow cytometric analysis of IL-4 expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies with Recombinant IL-2 Cat. No. 550069; 10 ng/ml and IL-4 Cat. No. 550067; 25 ng/ml . The cells were subsequently cultured 3 days with Recombinant IL-2 and IL-4. The cells were then stimulated with PMA Sigma Cat. No. P-8139 and Ionomycin Sigma Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 6 hours. The cells were harvested, washed, and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon BV605 Rat IgG1, kappa Isotype Control Cat. No. 562993; Left Panel or BD Horizon BV605 Rat Anti-Mouse IL-4 antibody Cat. No. 564007; Right Panel . Flow cytometric dot plots showing correlated expression of IL-4 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-4 expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies with Recombinant IL-2 Cat. No. 550069; 10 ng/ml and IL-4 Cat. No. 550067; 25 ng/ml . The cells were subsequently cultured 3 days with Recombinant IL-2 and IL-4. The cells were then stimulated with PMA Sigma Cat. No. P-8139 and Ionomycin Sigma Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 6 hours. The cells were harvested, washed, and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon BV605 Rat IgG1, kappa Isotype Control Cat. No. 562993; Left Panel or BD Horizon BV605 Rat Anti-Mouse IL-4 antibody Cat. No. 564007; Right Panel . Flow cytometric dot plots showing correlated expression of IL-4 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-4 expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies with Recombinant IL-2 Cat. No. 550069; 10 ng/ml and IL-4 Cat. No. 550067; 25 ng/ml . The cells were subsequently cultured 3 days with Recombinant IL-2 and IL-4. The cells were then stimulated with PMA Sigma Cat. No. P-8139 and Ionomycin Sigma Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 6 hours. The cells were harvested, washed, and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon BV605 Rat IgG1, kappa Isotype Control Cat. No. 562993; Left Panel or BD Horizon BV605 Rat Anti-Mouse IL-4 antibody Cat. No. 564007; Right Panel . Flow cytometric dot plots showing correlated expression of IL-4 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IL-4 expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies with Recombinant IL-2 Cat. No. 550069; 10 ng/ml and IL-4 Cat. No. 550067; 25 ng/ml . The cells were subsequently cultured 3 days with Recombinant IL-2 and IL-4. The cells were then stimulated with PMA Sigma Cat. No. P-8139 and Ionomycin Sigma Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 6 hours. The cells were harvested, washed, and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon BV605 Rat IgG1, kappa Isotype Control Cat. No. 562993; Left Panel or BD Horizon BV605 Rat Anti-Mouse IL-4 antibody Cat. No. 564007; Right Panel . Flow cytometric dot plots showing correlated expression of IL-4 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IL-4 expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies with Recombinant IL-2 Cat. No. 550069; 10 ng/ml and IL-4 Cat. No. 550067; 25 ng/ml . The cells were subsequently cultured 3 days with Recombinant IL-2 and IL-4. The cells were then stimulated with PMA Sigma Cat. No. P-8139 and Ionomycin Sigma Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 6 hours. The cells were harvested, washed, and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon BV605 Rat IgG1, kappa Isotype Control Cat. No. 562993; Left Panel or BD Horizon BV605 Rat Anti-Mouse IL-4 antibody Cat. No. 564007; Right Panel . Flow cytometric dot plots showing correlated expression of IL-4 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IL-4 expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured 2 days with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e Cat. No. 553057; 10 mug/ml for coating and soluble Purified NA/LE Hamster Anti-Mouse CD28 Cat. No. 553294; 2 mug/ml antibodies with Recombinant IL-2 Cat. No. 550069; 10 ng/ml and IL-4 Cat. No. 550067; 25 ng/ml . The cells were subsequently cultured 3 days with Recombinant IL-2 and IL-4. The cells were then stimulated with PMA Sigma Cat. No. P-8139 and Ionomycin Sigma Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 6 hours. The cells were harvested, washed, and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon BV605 Rat IgG1, kappa Isotype Control Cat. No. 562993; Left Panel or BD Horizon BV605 Rat Anti-Mouse IL-4 antibody Cat. No. 564007; Right Panel . Flow cytometric dot plots showing correlated expression of IL-4 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.