BD HorizonTM BV605 Rat Anti-Mouse TNF

Description

Two-color flow cytometric analysis of TNF expression by stimulated Mouse splenic leukocytes. Mouse splenocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 568375 and with either BD Horizon BV605 Rat IgG1, kappa Isotype Control Cat. No. 562993; Left Plot or BD Horizon BV605 Rat Anti-Mouse TNF antibody Cat. No. 569296/569297; Right Plot at 0.25 mug/test by using BD Biosciences Intracellular Cytokine Staining protocol. The pseudocolor density plot showing correlated expression of TNF or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TNF expression by stimulated Mouse splenic leukocytes. Mouse splenocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 568375 and with either BD Horizon BV605 Rat IgG1, kappa Isotype Control Cat. No. 562993; Left Plot or BD Horizon BV605 Rat Anti-Mouse TNF antibody Cat. No. 569296/569297; Right Plot at 0.25 mug/test by using BD Biosciences Intracellular Cytokine Staining protocol. The pseudocolor density plot showing correlated expression of TNF or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TNF expression by stimulated Mouse splenic leukocytes. Mouse splenocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 568375 and with either BD Horizon BV605 Rat IgG1, kappa Isotype Control Cat. No. 562993; Left Plot or BD Horizon BV605 Rat Anti-Mouse TNF antibody Cat. No. 569296/569297; Right Plot at 0.25 mug/test by using BD Biosciences Intracellular Cytokine Staining protocol. The pseudocolor density plot showing correlated expression of TNF or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of TNF expression by stimulated Mouse splenic leukocytes. Mouse splenocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 568375 and with either BD Horizon BV605 Rat IgG1, kappa Isotype Control Cat. No. 562993; Left Plot or BD Horizon BV605 Rat Anti-Mouse TNF antibody Cat. No. 569296/569297; Right Plot at 0.25 mug/test by using BD Biosciences Intracellular Cytokine Staining protocol. The pseudocolor density plot showing correlated expression of TNF or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of TNF expression by stimulated Mouse splenic leukocytes. Mouse splenocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 568375 and with either BD Horizon BV605 Rat IgG1, kappa Isotype Control Cat. No. 562993; Left Plot or BD Horizon BV605 Rat Anti-Mouse TNF antibody Cat. No. 569296/569297; Right Plot at 0.25 mug/test by using BD Biosciences Intracellular Cytokine Staining protocol. The pseudocolor density plot showing correlated expression of TNF or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TNF expression by stimulated Mouse splenic leukocytes. Mouse splenocytes were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139; 50 ng/ml and Ionomycin Sigma I-0634; 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with BD Horizon BUV395 Rat Anti-Mouse CD4 antibody Cat. No. 568375 and with either BD Horizon BV605 Rat IgG1, kappa Isotype Control Cat. No. 562993; Left Plot or BD Horizon BV605 Rat Anti-Mouse TNF antibody Cat. No. 569296/569297; Right Plot at 0.25 mug/test by using BD Biosciences Intracellular Cytokine Staining protocol. The pseudocolor density plot showing correlated expression of TNF or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.