BD HorizonTM BV650 Hamster Anti-Mouse CD3e

Description

Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553033/553032/561095 antibodies and either BD Horizon BV650 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 564388; Left Panel or BD Horizon BV650 Hamster Anti-Mouse CD3e antibody Cat. No. 564378; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometry System. Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553033/553032/561095 antibodies and either BD Horizon BV650 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 564388; Left Panel or BD Horizon BV650 Hamster Anti-Mouse CD3e antibody Cat. No. 564378; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometry System. Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553033/553032/561095 antibodies and either BD Horizon BV650 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 564388; Left Panel or BD Horizon BV650 Hamster Anti-Mouse CD3e antibody Cat. No. 564378; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometry System. Show More Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553033/553032/561095 antibodies and either BD Horizon BV650 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 564388; Left Panel or BD Horizon BV650 Hamster Anti-Mouse CD3e antibody Cat. No. 564378; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometry System. Show More Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553033/553032/561095 antibodies and either BD Horizon BV650 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 564388; Left Panel or BD Horizon BV650 Hamster Anti-Mouse CD3e antibody Cat. No. 564378; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometry System. Two-color flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 and PE Rat Anti-Mouse CD8a Cat. No. 553033/553032/561095 antibodies and either BD Horizon BV650 Armenian Hamster IgG1, kappa Isotype Control Cat. No. 564388; Left Panel or BD Horizon BV650 Hamster Anti-Mouse CD3e antibody Cat. No. 564378; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD3e or Ig Isotype control staining versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometry System.