BD HorizonTM BV650 Hamster Anti-Mouse KLRG1

Description

Multicolor flow cytometric analysis of KLRG1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Mouse Anti-Mouse NK-1.1 antibody Cat. No. 553165/557391/561046 and with either BD Horizon BV650 Hamster IgG2, kappa Isotype Control Cat. No. 563860; Left Panel or BD Horizon BV650 Hamster Anti-Mouse KLRG1 antibody Cat. No. 569548; Right Panel at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of KLRG1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Mouse Anti-Mouse NK-1.1 antibody Cat. No. 553165/557391/561046 and with either BD Horizon BV650 Hamster IgG2, kappa Isotype Control Cat. No. 563860; Left Panel or BD Horizon BV650 Hamster Anti-Mouse KLRG1 antibody Cat. No. 569548; Right Panel at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of KLRG1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Mouse Anti-Mouse NK-1.1 antibody Cat. No. 553165/557391/561046 and with either BD Horizon BV650 Hamster IgG2, kappa Isotype Control Cat. No. 563860; Left Panel or BD Horizon BV650 Hamster Anti-Mouse KLRG1 antibody Cat. No. 569548; Right Panel at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of KLRG1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Mouse Anti-Mouse NK-1.1 antibody Cat. No. 553165/557391/561046 and with either BD Horizon BV650 Hamster IgG2, kappa Isotype Control Cat. No. 563860; Left Panel or BD Horizon BV650 Hamster Anti-Mouse KLRG1 antibody Cat. No. 569548; Right Panel at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of KLRG1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Mouse Anti-Mouse NK-1.1 antibody Cat. No. 553165/557391/561046 and with either BD Horizon BV650 Hamster IgG2, kappa Isotype Control Cat. No. 563860; Left Panel or BD Horizon BV650 Hamster Anti-Mouse KLRG1 antibody Cat. No. 569548; Right Panel at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of KLRG1 expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Mouse Anti-Mouse NK-1.1 antibody Cat. No. 553165/557391/561046 and with either BD Horizon BV650 Hamster IgG2, kappa Isotype Control Cat. No. 563860; Left Panel or BD Horizon BV650 Hamster Anti-Mouse KLRG1 antibody Cat. No. 569548; Right Panel at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of KLRG1 or Ig Isotype control staining versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.