
569617 ·
BDB Bioscience · Cat: 569617
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multiparameter flow cytometric analysis of CD1c expression on Human peripheral blood lymphocytes Top Plots and leucocyte populations Bottom Plots . Human whole blood was stained with PE Mouse Anti-Human CD19 antibody Cat. No. 555413/561741; Lower Plots and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat. No. 563231; Left Plots or BD Horizon BV650 Mouse Anti-Human CD1c antibody Cat. No. 569617; Right Plots at 1.0 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots: The bivariate pseudocolor density plot showing the correlated expression of CD1c or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: The bivariate pseudocolor density plot showing CD1c expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD1c expression on Human peripheral blood lymphocytes Top Plots and leucocyte populations Bottom Plots . Human whole blood was stained with PE Mouse Anti-Human CD19 antibody Cat. No. 555413/561741; Lower Plots and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat. No. 563231; Left Plots or BD Horizon BV650 Mouse Anti-Human CD1c antibody Cat. No. 569617; Right Plots at 1.0 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots: The bivariate pseudocolor density plot showing the correlated expression of CD1c or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: The bivariate pseudocolor density plot showing CD1c expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD1c expression on Human peripheral blood lymphocytes Top Plots and leucocyte populations Bottom Plots . Human whole blood was stained with PE Mouse Anti-Human CD19 antibody Cat. No. 555413/561741; Lower Plots and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat. No. 563231; Left Plots or BD Horizon BV650 Mouse Anti-Human CD1c antibody Cat. No. 569617; Right Plots at 1.0 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Top Plots: The bivariate pseudocolor density plot showing the correlated expression of CD1c or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: The bivariate pseudocolor density plot showing CD1c expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.