
563719 · 100 Tests
BDB Bioscience · Cat: 563719
Reach 3+ verified distributors
Manufacturer product page
bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left Panel: Whole blood was stained with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat. No. 563231; dashed line histogram or BD Horizon BV650 Mouse Anti-Human CD25 antibody Cat. No. 563718/563719; solid line histogram . The erythrocytes were subsequently lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Right Panel: Phytohemagglutinin-stimulated 3 days human peripheral blood mononuclear cells were stained with either BD Horizon BV650 Mouse Anti-Human CD25 antibody solid line histogram or with BD Horizon BV650 Mouse IgG1, kappa Isotype Control dashed line histogram . The fluorescence histograms showing CD25 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes Left Panel or lymphoblasts Right Panel . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left Panel: Whole blood was stained with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat. No. 563231; dashed line histogram or BD Horizon BV650 Mouse Anti-Human CD25 antibody Cat. No. 563718/563719; solid line histogram . The erythrocytes were subsequently lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Right Panel: Phytohemagglutinin-stimulated 3 days human peripheral blood mononuclear cells were stained with either BD Horizon BV650 Mouse Anti-Human CD25 antibody solid line histogram or with BD Horizon BV650 Mouse IgG1, kappa Isotype Control dashed line histogram . The fluorescence histograms showing CD25 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes Left Panel or lymphoblasts Right Panel . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left Panel: Whole blood was stained with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat. No. 563231; dashed line histogram or BD Horizon BV650 Mouse Anti-Human CD25 antibody Cat. No. 563718/563719; solid line histogram . The erythrocytes were subsequently lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Right Panel: Phytohemagglutinin-stimulated 3 days human peripheral blood mononuclear cells were stained with either BD Horizon BV650 Mouse Anti-Human CD25 antibody solid line histogram or with BD Horizon BV650 Mouse IgG1, kappa Isotype Control dashed line histogram . The fluorescence histograms showing CD25 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes Left Panel or lymphoblasts Right Panel . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left Panel: Whole blood was stained with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat. No. 563231; dashed line histogram or BD Horizon BV650 Mouse Anti-Human CD25 antibody Cat. No. 563718/563719; solid line histogram . The erythrocytes were subsequently lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Right Panel: Phytohemagglutinin-stimulated 3 days human peripheral blood mononuclear cells were stained with either BD Horizon BV650 Mouse Anti-Human CD25 antibody solid line histogram or with BD Horizon BV650 Mouse IgG1, kappa Isotype Control dashed line histogram . The fluorescence histograms showing CD25 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes Left Panel or lymphoblasts Right Panel . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left Panel: Whole blood was stained with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat. No. 563231; dashed line histogram or BD Horizon BV650 Mouse Anti-Human CD25 antibody Cat. No. 563718/563719; solid line histogram . The erythrocytes were subsequently lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Right Panel: Phytohemagglutinin-stimulated 3 days human peripheral blood mononuclear cells were stained with either BD Horizon BV650 Mouse Anti-Human CD25 antibody solid line histogram or with BD Horizon BV650 Mouse IgG1, kappa Isotype Control dashed line histogram . The fluorescence histograms showing CD25 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes Left Panel or lymphoblasts Right Panel . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated human peripheral blood lymphocytes. Left Panel: Whole blood was stained with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat. No. 563231; dashed line histogram or BD Horizon BV650 Mouse Anti-Human CD25 antibody Cat. No. 563718/563719; solid line histogram . The erythrocytes were subsequently lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Right Panel: Phytohemagglutinin-stimulated 3 days human peripheral blood mononuclear cells were stained with either BD Horizon BV650 Mouse Anti-Human CD25 antibody solid line histogram or with BD Horizon BV650 Mouse IgG1, kappa Isotype Control dashed line histogram . The fluorescence histograms showing CD25 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes Left Panel or lymphoblasts Right Panel . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.