BD HorizonTM BV650 Mouse Anti-Human CD279 (PD-1)

Description

Two-color flow cytometric analysis of CD279 PD-1 expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808/561809 and either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat. No. 563231; Left Panel or BD Horizon BV650 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 564324; Right Panel . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric dot plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 for derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD279 PD-1 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin-stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV650 Mouse Anti-Human CD279 PD-1 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD279 PD-1 expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808/561809 and either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat. No. 563231; Left Panel or BD Horizon BV650 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 564324; Right Panel . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric dot plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 for derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD279 PD-1 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin-stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV650 Mouse Anti-Human CD279 PD-1 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD279 PD-1 expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808/561809 and either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat. No. 563231; Left Panel or BD Horizon BV650 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 564324; Right Panel . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Two-color flow cytometric dot plots showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 for derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD279 PD-1 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin-stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon BV650 Mouse Anti-Human CD279 PD-1 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.