BD HorizonTM BV650 Mouse Anti-Human IFN-gamma

Description

Two-color flow cytometric analysis of IFN-gamma expression in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat No. 563231, Left Panel or with the BD Horizon BV650 Mouse Anti-Human IFN-gamma antibody Cat No. 563416, Right Panel . Two-color flow cytometric dot plots showing the expression of IFN-gamma or Ig Isotype Control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IFN-gamma expression in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat No. 563231, Left Panel or with the BD Horizon BV650 Mouse Anti-Human IFN-gamma antibody Cat No. 563416, Right Panel . Two-color flow cytometric dot plots showing the expression of IFN-gamma or Ig Isotype Control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IFN-gamma expression in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat No. 563231, Left Panel or with the BD Horizon BV650 Mouse Anti-Human IFN-gamma antibody Cat No. 563416, Right Panel . Two-color flow cytometric dot plots showing the expression of IFN-gamma or Ig Isotype Control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IFN-gamma expression in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat No. 563231, Left Panel or with the BD Horizon BV650 Mouse Anti-Human IFN-gamma antibody Cat No. 563416, Right Panel . Two-color flow cytometric dot plots showing the expression of IFN-gamma or Ig Isotype Control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of IFN-gamma expression in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat No. 563231, Left Panel or with the BD Horizon BV650 Mouse Anti-Human IFN-gamma antibody Cat No. 563416, Right Panel . Two-color flow cytometric dot plots showing the expression of IFN-gamma or Ig Isotype Control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of IFN-gamma expression in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with APC Mouse Anti-Human CD3 antibody Cat. No. 561811/555335/561810 and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat No. 563231, Left Panel or with the BD Horizon BV650 Mouse Anti-Human IFN-gamma antibody Cat No. 563416, Right Panel . Two-color flow cytometric dot plots showing the expression of IFN-gamma or Ig Isotype Control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.