BD HorizonTM BV650 Mouse Anti-Human PD-L1 (CD274)

Description

Flow cytometric analysis of PD-L1 CD274 expression on activated Human lymphocytes. Human peripheral blood mononuclear cells were stimulated with Phytohemagglutinin PHA for 3 days and stained with either BD Horizon BV650 Mouse IgG2b, kappa Isotype Control Cat. No. 563437; dotted line histogram or BD Horizon BV650 Mouse Anti-Human PD-L1 CD274 antibody Cat. No. 568321/568322; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of PD-L1 CD274 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of PD-L1 CD274 expression on activated Human lymphocytes. Human peripheral blood mononuclear cells were stimulated with Phytohemagglutinin PHA for 3 days and stained with either BD Horizon BV650 Mouse IgG2b, kappa Isotype Control Cat. No. 563437; dotted line histogram or BD Horizon BV650 Mouse Anti-Human PD-L1 CD274 antibody Cat. No. 568321/568322; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of PD-L1 CD274 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of PD-L1 CD274 expression on activated Human lymphocytes. Human peripheral blood mononuclear cells were stimulated with Phytohemagglutinin PHA for 3 days and stained with either BD Horizon BV650 Mouse IgG2b, kappa Isotype Control Cat. No. 563437; dotted line histogram or BD Horizon BV650 Mouse Anti-Human PD-L1 CD274 antibody Cat. No. 568321/568322; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of PD-L1 CD274 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.