
569510 ·
BDB Bioscience · Cat: 569510
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Description
Two-color flow cytometric analysis of TCR gammadelta expressed on Human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat No. 563231; Left Plot or BD Horizon BV650 Mouse Anti-Human TCR gammadelta antibody Cat No. 569510; Right Plot at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR gammadelta or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TCR gammadelta expressed on Human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat No. 563231; Left Plot or BD Horizon BV650 Mouse Anti-Human TCR gammadelta antibody Cat No. 569510; Right Plot at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR gammadelta or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TCR gammadelta expressed on Human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat No. 563231; Left Plot or BD Horizon BV650 Mouse Anti-Human TCR gammadelta antibody Cat No. 569510; Right Plot at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR gammadelta or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of TCR gammadelta expressed on Human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat No. 563231; Left Plot or BD Horizon BV650 Mouse Anti-Human TCR gammadelta antibody Cat No. 569510; Right Plot at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR gammadelta or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of TCR gammadelta expressed on Human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat No. 563231; Left Plot or BD Horizon BV650 Mouse Anti-Human TCR gammadelta antibody Cat No. 569510; Right Plot at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR gammadelta or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TCR gammadelta expressed on Human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either BD Horizon BV650 Mouse IgG1, kappa Isotype Control Cat No. 563231; Left Plot or BD Horizon BV650 Mouse Anti-Human TCR gammadelta antibody Cat No. 569510; Right Plot at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of TCR gammadelta or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.