BD HorizonTM BV650 Mouse Anti-Ki-67

Description

Two-color flow cytometric analysis of Ki-67 expression by noncycling human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling peripheral blood mononuclear cells PBMC or proliferating cells from the human MOLT-4 ATCC CRL-1582 cell line were permeabilized and fixed with 70 % ice cold ethanol. The cells were washed twice with Stain Buffer FBS Cat. No. 554656 , stained with BD Horizon BV650 Mouse Anti-Ki-67 antibody Cat. No. 563757 and counterstained with DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of PBMC or intact MOLT-4 cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of Ki-67 expression by noncycling human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling peripheral blood mononuclear cells PBMC or proliferating cells from the human MOLT-4 ATCC CRL-1582 cell line were permeabilized and fixed with 70 % ice cold ethanol. The cells were washed twice with Stain Buffer FBS Cat. No. 554656 , stained with BD Horizon BV650 Mouse Anti-Ki-67 antibody Cat. No. 563757 and counterstained with DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of PBMC or intact MOLT-4 cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of Ki-67 expression by noncycling human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling peripheral blood mononuclear cells PBMC or proliferating cells from the human MOLT-4 ATCC CRL-1582 cell line were permeabilized and fixed with 70 % ice cold ethanol. The cells were washed twice with Stain Buffer FBS Cat. No. 554656 , stained with BD Horizon BV650 Mouse Anti-Ki-67 antibody Cat. No. 563757 and counterstained with DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of PBMC or intact MOLT-4 cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.