BD HorizonTM BV650 Mouse Anti-Mouse RORgammat

Description

Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies, and fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with BD Horizon BV650 Mouse Anti-Mouse RORgammat antibody Cat. No. 564722 . The overlapping histograms showing RORgammat expression were derived from CD4+CD8- single-positive dashed line histogram or CD4+CD8+ double-positive solid line histogram gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies, and fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with BD Horizon BV650 Mouse Anti-Mouse RORgammat antibody Cat. No. 564722 . The overlapping histograms showing RORgammat expression were derived from CD4+CD8- single-positive dashed line histogram or CD4+CD8+ double-positive solid line histogram gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies, and fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with BD Horizon BV650 Mouse Anti-Mouse RORgammat antibody Cat. No. 564722 . The overlapping histograms showing RORgammat expression were derived from CD4+CD8- single-positive dashed line histogram or CD4+CD8+ double-positive solid line histogram gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.