BD HorizonTM BV650 Rat Anti-Human IL-2

Description

Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 , washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat No. 555333/561808/561809 and either BD Horizon BV650 Rat IgG2a, kappa Isotype Control Cat No. 563236; Left Panel or BD Horizon BV650 Rat Anti-Human IL-2 antibody Cat No. 564166; Right Panel using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric contour plots showing correlated expression of IL-2 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 , washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat No. 555333/561808/561809 and either BD Horizon BV650 Rat IgG2a, kappa Isotype Control Cat No. 563236; Left Panel or BD Horizon BV650 Rat Anti-Human IL-2 antibody Cat No. 564166; Right Panel using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric contour plots showing correlated expression of IL-2 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 , washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat No. 555333/561808/561809 and either BD Horizon BV650 Rat IgG2a, kappa Isotype Control Cat No. 563236; Left Panel or BD Horizon BV650 Rat Anti-Human IL-2 antibody Cat No. 564166; Right Panel using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric contour plots showing correlated expression of IL-2 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 , washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat No. 555333/561808/561809 and either BD Horizon BV650 Rat IgG2a, kappa Isotype Control Cat No. 563236; Left Panel or BD Horizon BV650 Rat Anti-Human IL-2 antibody Cat No. 564166; Right Panel using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric contour plots showing correlated expression of IL-2 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 , washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat No. 555333/561808/561809 and either BD Horizon BV650 Rat IgG2a, kappa Isotype Control Cat No. 563236; Left Panel or BD Horizon BV650 Rat Anti-Human IL-2 antibody Cat No. 564166; Right Panel using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric contour plots showing correlated expression of IL-2 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two color flow cytometric analysis of IL-2 expression in activated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were fixed with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 , washed and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat No. 555333/561808/561809 and either BD Horizon BV650 Rat IgG2a, kappa Isotype Control Cat No. 563236; Left Panel or BD Horizon BV650 Rat Anti-Human IL-2 antibody Cat No. 564166; Right Panel using BD Biosciences Intracellular Cytokine Staining Protocol. The two-color flow cytometric contour plots showing correlated expression of IL-2 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.