BD HorizonTM BV650 Rat Anti-Mouse CD279 (PD-1)

Description

Flow cytometric analysis of CD279 PD-1 expression on resting and activated viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were either not activated Left Plot or activated Right Plot by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 567114/567115 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . Cells were then stained with either BD Horizon BV650 Rat IgG2a, kappa Isotype Control Cat. No. 563236; dashed line histograms or BD Horizon BV650 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 568616/568617; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD279 PD-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were either not activated Left Plot or activated Right Plot by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 567114/567115 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . Cells were then stained with either BD Horizon BV650 Rat IgG2a, kappa Isotype Control Cat. No. 563236; dashed line histograms or BD Horizon BV650 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 568616/568617; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD279 PD-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were either not activated Left Plot or activated Right Plot by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 567114/567115 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . Cells were then stained with either BD Horizon BV650 Rat IgG2a, kappa Isotype Control Cat. No. 563236; dashed line histograms or BD Horizon BV650 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 568616/568617; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD279 PD-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD279 PD-1 expression on resting and activated viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were either not activated Left Plot or activated Right Plot by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 567114/567115 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . Cells were then stained with either BD Horizon BV650 Rat IgG2a, kappa Isotype Control Cat. No. 563236; dashed line histograms or BD Horizon BV650 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 568616/568617; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD279 PD-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD279 PD-1 expression on resting and activated viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were either not activated Left Plot or activated Right Plot by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 567114/567115 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . Cells were then stained with either BD Horizon BV650 Rat IgG2a, kappa Isotype Control Cat. No. 563236; dashed line histograms or BD Horizon BV650 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 568616/568617; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD279 PD-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD279 PD-1 expression on resting and activated viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were either not activated Left Plot or activated Right Plot by culture with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 567114/567115 for 3 days 37C . The cells were harvested, washed and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . Cells were then stained with either BD Horizon BV650 Rat IgG2a, kappa Isotype Control Cat. No. 563236; dashed line histograms or BD Horizon BV650 Rat Anti-Mouse CD279 PD-1 antibody Cat. No. 568616/568617; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD279 PD-1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.