BD HorizonTM BV650 Rat Anti-Mouse CD44

Description

Multiparameter flow cytometric analysis of CD44 expression on viable Mouse bone marrow cells. BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV650 Rat IgG2b, kappa Isotype Control Cat. No. 563233; Left Plot or BD Horizon BV650 Rat Anti-Mouse CD44 antibody Cat. No. 569707; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD44 expression or Ig Isotype control staining was derived from DAPI-negative gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD44 expression on viable Mouse bone marrow cells. BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV650 Rat IgG2b, kappa Isotype Control Cat. No. 563233; Left Plot or BD Horizon BV650 Rat Anti-Mouse CD44 antibody Cat. No. 569707; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD44 expression or Ig Isotype control staining was derived from DAPI-negative gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD44 expression on viable Mouse bone marrow cells. BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV650 Rat IgG2b, kappa Isotype Control Cat. No. 563233; Left Plot or BD Horizon BV650 Rat Anti-Mouse CD44 antibody Cat. No. 569707; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD44 expression or Ig Isotype control staining was derived from DAPI-negative gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD44 expression on viable Mouse bone marrow cells. BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV650 Rat IgG2b, kappa Isotype Control Cat. No. 563233; Left Plot or BD Horizon BV650 Rat Anti-Mouse CD44 antibody Cat. No. 569707; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD44 expression or Ig Isotype control staining was derived from DAPI-negative gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD44 expression on viable Mouse bone marrow cells. BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV650 Rat IgG2b, kappa Isotype Control Cat. No. 563233; Left Plot or BD Horizon BV650 Rat Anti-Mouse CD44 antibody Cat. No. 569707; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD44 expression or Ig Isotype control staining was derived from DAPI-negative gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD44 expression on viable Mouse bone marrow cells. BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV650 Rat IgG2b, kappa Isotype Control Cat. No. 563233; Left Plot or BD Horizon BV650 Rat Anti-Mouse CD44 antibody Cat. No. 569707; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD44 expression or Ig Isotype control staining was derived from DAPI-negative gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.