

563994 ·
BDB Bioscience · Cat: 563994
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Description
Two-color flow cytometric analysis of gammadelta TCR expression on mouse peripheral T lymphocytes. Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD3 Molecular Complex Cat. No. 555274/561798 and either BV711 Hamster IgG2, kappa Isotype Control Cat. No. 563989; left panel or BV711 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 563994, right panel . Two-color flow cytometric dot plots showing the correlated expression of gammadelta T-Cell Receptor or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD LSR II. Two-color flow cytometric analysis of gammadelta TCR expression on mouse peripheral T lymphocytes. Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD3 Molecular Complex Cat. No. 555274/561798 and either BV711 Hamster IgG2, kappa Isotype Control Cat. No. 563989; left panel or BV711 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 563994, right panel . Two-color flow cytometric dot plots showing the correlated expression of gammadelta T-Cell Receptor or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD LSR II. Two-color flow cytometric analysis of gammadelta TCR expression on mouse peripheral T lymphocytes. Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD3 Molecular Complex Cat. No. 555274/561798 and either BV711 Hamster IgG2, kappa Isotype Control Cat. No. 563989; left panel or BV711 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 563994, right panel . Two-color flow cytometric dot plots showing the correlated expression of gammadelta T-Cell Receptor or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD LSR II. Show More Two-color flow cytometric analysis of gammadelta TCR expression on mouse peripheral T lymphocytes. Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD3 Molecular Complex Cat. No. 555274/561798 and either BV711 Hamster IgG2, kappa Isotype Control Cat. No. 563989; left panel or BV711 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 563994, right panel . Two-color flow cytometric dot plots showing the correlated expression of gammadelta T-Cell Receptor or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD LSR II. Show More Two-color flow cytometric analysis of gammadelta TCR expression on mouse peripheral T lymphocytes. Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD3 Molecular Complex Cat. No. 555274/561798 and either BV711 Hamster IgG2, kappa Isotype Control Cat. No. 563989; left panel or BV711 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 563994, right panel . Two-color flow cytometric dot plots showing the correlated expression of gammadelta T-Cell Receptor or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD LSR II. Two-color flow cytometric analysis of gammadelta TCR expression on mouse peripheral T lymphocytes. Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD3 Molecular Complex Cat. No. 555274/561798 and either BV711 Hamster IgG2, kappa Isotype Control Cat. No. 563989; left panel or BV711 Hamster Anti-Mouse gammadelta T-Cell Receptor Cat. No. 563994, right panel . Two-color flow cytometric dot plots showing the correlated expression of gammadelta T-Cell Receptor or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD LSR II.







