BD HorizonTM BV711 Mouse Anti-Human CCR7 (CD197)

Description

Flow cytometric analysis of CCR7 CD197 on human peripheral blood leukocytes and lymphocytes. Whole blood was stained with Alexa Fluor647 Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 557707 , PE Mouse Anti-Human CD45RA clone HI100, Cat. No. 555489 or 561883 , and either BD Horizon BV711 Mouse IgG1, kappa Isotype Control Cat. No. 563044 or BD Horizon BV711 Mouse Anti-Human CCR7 CD197 Cat. No. 566752 or 566753 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer and FlowJo software. Plots 1 and 2: The contour plots showing Ig isotype control or CCR7 CD197 staining versus side light-scatter were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plots 3 and 4: The contour plots showing Ig isotype control or CCR7 CD197 staining versus CD45RA were derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive CD45RA-negative lymphocytes. Flow cytometric analysis of CCR7 CD197 on human peripheral blood leukocytes and lymphocytes. Whole blood was stained with Alexa Fluor647 Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 557707 , PE Mouse Anti-Human CD45RA clone HI100, Cat. No. 555489 or 561883 , and either BD Horizon BV711 Mouse IgG1, kappa Isotype Control Cat. No. 563044 or BD Horizon BV711 Mouse Anti-Human CCR7 CD197 Cat. No. 566752 or 566753 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer and FlowJo software. Plots 1 and 2: The contour plots showing Ig isotype control or CCR7 CD197 staining versus side light-scatter were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plots 3 and 4: The contour plots showing Ig isotype control or CCR7 CD197 staining versus CD45RA were derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive CD45RA-negative lymphocytes. Show More Flow cytometric analysis of CCR7 CD197 on human peripheral blood leukocytes and lymphocytes. Whole blood was stained with Alexa Fluor647 Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 557707 , PE Mouse Anti-Human CD45RA clone HI100, Cat. No. 555489 or 561883 , and either BD Horizon BV711 Mouse IgG1, kappa Isotype Control Cat. No. 563044 or BD Horizon BV711 Mouse Anti-Human CCR7 CD197 Cat. No. 566752 or 566753 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer and FlowJo software. Plots 1 and 2: The contour plots showing Ig isotype control or CCR7 CD197 staining versus side light-scatter were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plots 3 and 4: The contour plots showing Ig isotype control or CCR7 CD197 staining versus CD45RA were derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive CD45RA-negative lymphocytes.