

563680 ·
BDB Bioscience · Cat: 563680
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood was stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , APC Mouse Anti-Human CD25 Cat. No. 555434/560987/561399 , PE Anti-Human CD127 Cat No. 557938/561028 antibodies, and either BD Horizon BV711 Mouse IgG2b, kappa Isotype Control Cat. No. 563125; dashed line histogram or BD Horizon BV711 Mouse Anti-Human CD39 antibody Cat. No. 563680; solid line histogram Left Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The fluorescence histograms were derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Right Panel with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood was stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , APC Mouse Anti-Human CD25 Cat. No. 555434/560987/561399 , PE Anti-Human CD127 Cat No. 557938/561028 antibodies, and either BD Horizon BV711 Mouse IgG2b, kappa Isotype Control Cat. No. 563125; dashed line histogram or BD Horizon BV711 Mouse Anti-Human CD39 antibody Cat. No. 563680; solid line histogram Left Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The fluorescence histograms were derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Right Panel with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood was stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , APC Mouse Anti-Human CD25 Cat. No. 555434/560987/561399 , PE Anti-Human CD127 Cat No. 557938/561028 antibodies, and either BD Horizon BV711 Mouse IgG2b, kappa Isotype Control Cat. No. 563125; dashed line histogram or BD Horizon BV711 Mouse Anti-Human CD39 antibody Cat. No. 563680; solid line histogram Left Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The fluorescence histograms were derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Right Panel with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood was stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , APC Mouse Anti-Human CD25 Cat. No. 555434/560987/561399 , PE Anti-Human CD127 Cat No. 557938/561028 antibodies, and either BD Horizon BV711 Mouse IgG2b, kappa Isotype Control Cat. No. 563125; dashed line histogram or BD Horizon BV711 Mouse Anti-Human CD39 antibody Cat. No. 563680; solid line histogram Left Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The fluorescence histograms were derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Right Panel with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood was stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , APC Mouse Anti-Human CD25 Cat. No. 555434/560987/561399 , PE Anti-Human CD127 Cat No. 557938/561028 antibodies, and either BD Horizon BV711 Mouse IgG2b, kappa Isotype Control Cat. No. 563125; dashed line histogram or BD Horizon BV711 Mouse Anti-Human CD39 antibody Cat. No. 563680; solid line histogram Left Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The fluorescence histograms were derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Right Panel with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD39 expression on peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood was stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842 , APC Mouse Anti-Human CD25 Cat. No. 555434/560987/561399 , PE Anti-Human CD127 Cat No. 557938/561028 antibodies, and either BD Horizon BV711 Mouse IgG2b, kappa Isotype Control Cat. No. 563125; dashed line histogram or BD Horizon BV711 Mouse Anti-Human CD39 antibody Cat. No. 563680; solid line histogram Left Panel . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The fluorescence histograms were derived from CD4+CD25+CD127low gated events ie, cells with a Regulatory T cell immunophenotype; Right Panel with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.