BD HorizonTM BV711 Mouse Anti-Human CD86 (B7-2)

Description

Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon BV711 Mouse IgG2b, kappa Isotype Control Cat. No. 563125; Left Plot or BD Horizon BV711 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 569383/569384; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either BD Horizon BV711 Mouse IgG2b, kappa Isotype Control Cat. No. 563125; dashed line histogram or BD Horizon BV711 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 569383/569384; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon BV711 Mouse IgG2b, kappa Isotype Control Cat. No. 563125; Left Plot or BD Horizon BV711 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 569383/569384; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either BD Horizon BV711 Mouse IgG2b, kappa Isotype Control Cat. No. 563125; dashed line histogram or BD Horizon BV711 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 569383/569384; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon BV711 Mouse IgG2b, kappa Isotype Control Cat. No. 563125; Left Plot or BD Horizon BV711 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 569383/569384; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either BD Horizon BV711 Mouse IgG2b, kappa Isotype Control Cat. No. 563125; dashed line histogram or BD Horizon BV711 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 569383/569384; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.