BD HorizonTM BV711 Mouse Anti-Ki-67

Description

Two-color flow cytometric analysis of Ki-67 expression by noncycling human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling peripheral blood mononuclear cells PBMC or proliferating cells from the human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line were permeabilized and fixed with 70 % ice cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BV711 Mouse Anti-Ki-67 antibody Cat. No. 563755 and counterstained with BD Pharmingen DAPI Solution Cat. No. 564907 to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of PBMC Left Panel or intact MOLT-4 cells stained with BV711 Mouse IgG1 isotype control Middle Panel or BV711 Mouse Anti- Ki-67 Right Panel . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of Ki-67 expression by noncycling human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling peripheral blood mononuclear cells PBMC or proliferating cells from the human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line were permeabilized and fixed with 70 % ice cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BV711 Mouse Anti-Ki-67 antibody Cat. No. 563755 and counterstained with BD Pharmingen DAPI Solution Cat. No. 564907 to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of PBMC Left Panel or intact MOLT-4 cells stained with BV711 Mouse IgG1 isotype control Middle Panel or BV711 Mouse Anti- Ki-67 Right Panel . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of Ki-67 expression by noncycling human peripheral blood mononuclear cells or proliferating MOLT-4 cells. Noncycling peripheral blood mononuclear cells PBMC or proliferating cells from the human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line were permeabilized and fixed with 70 % ice cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BV711 Mouse Anti-Ki-67 antibody Cat. No. 563755 and counterstained with BD Pharmingen DAPI Solution Cat. No. 564907 to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of DAPI staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of PBMC Left Panel or intact MOLT-4 cells stained with BV711 Mouse IgG1 isotype control Middle Panel or BV711 Mouse Anti- Ki-67 Right Panel . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.