BD HorizonTM BV711 Rat Anti-Mouse CD115 (CSF-1R)

Description

Multicolor flow cytometric analysis of CD115 CSF-1R expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD11b antibody Cat. No. 562605 and either BD Horizon BV711 Rat IgG1, kappa Isotype Control Cat. No. 563283; Left Plot or BD Horizon BV711 Rat Anti-Mouse CD115 CSF-1R antibody Cat. No. 567460; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD115 CSF-1R or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scattering characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD115 CSF-1R expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD11b antibody Cat. No. 562605 and either BD Horizon BV711 Rat IgG1, kappa Isotype Control Cat. No. 563283; Left Plot or BD Horizon BV711 Rat Anti-Mouse CD115 CSF-1R antibody Cat. No. 567460; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD115 CSF-1R or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scattering characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD115 CSF-1R expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD11b antibody Cat. No. 562605 and either BD Horizon BV711 Rat IgG1, kappa Isotype Control Cat. No. 563283; Left Plot or BD Horizon BV711 Rat Anti-Mouse CD115 CSF-1R antibody Cat. No. 567460; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD115 CSF-1R or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scattering characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD115 CSF-1R expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD11b antibody Cat. No. 562605 and either BD Horizon BV711 Rat IgG1, kappa Isotype Control Cat. No. 563283; Left Plot or BD Horizon BV711 Rat Anti-Mouse CD115 CSF-1R antibody Cat. No. 567460; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD115 CSF-1R or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scattering characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD115 CSF-1R expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD11b antibody Cat. No. 562605 and either BD Horizon BV711 Rat IgG1, kappa Isotype Control Cat. No. 563283; Left Plot or BD Horizon BV711 Rat Anti-Mouse CD115 CSF-1R antibody Cat. No. 567460; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD115 CSF-1R or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scattering characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD115 CSF-1R expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BV421 Rat Anti-Mouse CD11b antibody Cat. No. 562605 and either BD Horizon BV711 Rat IgG1, kappa Isotype Control Cat. No. 563283; Left Plot or BD Horizon BV711 Rat Anti-Mouse CD115 CSF-1R antibody Cat. No. 567460; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD115 CSF-1R or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scattering characteristics of viable 7-AAD-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.