
565490 ·
BDB Bioscience · Cat: 565490
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Two-color flow cytometric analysis of CD127 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either BD Horizon BV711 Rat IgG2b, kappa Isotype Control Cat. No. 563045; Left Panel or BD Horizon BV711 Rat Anti-Mouse CD127 antibody Cat. No. 565490; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD127 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD127 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either BD Horizon BV711 Rat IgG2b, kappa Isotype Control Cat. No. 563045; Left Panel or BD Horizon BV711 Rat Anti-Mouse CD127 antibody Cat. No. 565490; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD127 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD127 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either BD Horizon BV711 Rat IgG2b, kappa Isotype Control Cat. No. 563045; Left Panel or BD Horizon BV711 Rat Anti-Mouse CD127 antibody Cat. No. 565490; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD127 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD127 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either BD Horizon BV711 Rat IgG2b, kappa Isotype Control Cat. No. 563045; Left Panel or BD Horizon BV711 Rat Anti-Mouse CD127 antibody Cat. No. 565490; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD127 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD127 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either BD Horizon BV711 Rat IgG2b, kappa Isotype Control Cat. No. 563045; Left Panel or BD Horizon BV711 Rat Anti-Mouse CD127 antibody Cat. No. 565490; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD127 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD127 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either BD Horizon BV711 Rat IgG2b, kappa Isotype Control Cat. No. 563045; Left Panel or BD Horizon BV711 Rat Anti-Mouse CD127 antibody Cat. No. 565490; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD127 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.