
563050 ·
BDB Bioscience · Cat: 563050
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multicolor flow cytometric analysis of CD4 expressed on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BV711 Rat IgG2b, kappa Isotype Control Cat. No. 563045, Left Plot or BD Horizon BV711 Rat Anti-Mouse CD4 antibody Cat. No. 563050, Right Plot at 0.125 mug/test. Two-color flow cytometric dot plots show the correlated expression patterns of CD4 or Ig Isotype control staining versus CD3 for gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD4 expressed on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BV711 Rat IgG2b, kappa Isotype Control Cat. No. 563045, Left Plot or BD Horizon BV711 Rat Anti-Mouse CD4 antibody Cat. No. 563050, Right Plot at 0.125 mug/test. Two-color flow cytometric dot plots show the correlated expression patterns of CD4 or Ig Isotype control staining versus CD3 for gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD4 expressed on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 and either BD Horizon BV711 Rat IgG2b, kappa Isotype Control Cat. No. 563045, Left Plot or BD Horizon BV711 Rat Anti-Mouse CD4 antibody Cat. No. 563050, Right Plot at 0.125 mug/test. Two-color flow cytometric dot plots show the correlated expression patterns of CD4 or Ig Isotype control staining versus CD3 for gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.