BD HorizonTM BV711 Rat Anti-Mouse IgG1

Description

Flow cytometric analysis of CD22.2 expression by mouse splenocytes using BD Horizon BV711 Rat Anti-Mouse IgG1 as a second step. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Purified Mouse IgG1, kappa Anti-Mouse CD22.2 antibody clone Cy34.1; solid line histogram or with no antibody for background staining control; dashed line histogram . After washing, the cells were stained with BD Horizon BV711 Rat Anti-Mouse IgG1 antibody Cat. No. 565786 as the second step. The fluorescence histogram showing CD22.2 expression or control staining as detected by BD Horizon BV711 Rat Anti-Mouse IgG1 antibody was derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD22.2 expression by mouse splenocytes using BD Horizon BV711 Rat Anti-Mouse IgG1 as a second step. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Purified Mouse IgG1, kappa Anti-Mouse CD22.2 antibody clone Cy34.1; solid line histogram or with no antibody for background staining control; dashed line histogram . After washing, the cells were stained with BD Horizon BV711 Rat Anti-Mouse IgG1 antibody Cat. No. 565786 as the second step. The fluorescence histogram showing CD22.2 expression or control staining as detected by BD Horizon BV711 Rat Anti-Mouse IgG1 antibody was derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD22.2 expression by mouse splenocytes using BD Horizon BV711 Rat Anti-Mouse IgG1 as a second step. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Purified Mouse IgG1, kappa Anti-Mouse CD22.2 antibody clone Cy34.1; solid line histogram or with no antibody for background staining control; dashed line histogram . After washing, the cells were stained with BD Horizon BV711 Rat Anti-Mouse IgG1 antibody Cat. No. 565786 as the second step. The fluorescence histogram showing CD22.2 expression or control staining as detected by BD Horizon BV711 Rat Anti-Mouse IgG1 antibody was derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.