BD HorizonTM BV750 Mouse Anti-Human CD137 (4-1BB)

Description

Flow cytometric analysis of CD137 4-1BB expression on stimulated Human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either BD Horizon BV750 Mouse IgG1, kappa Isotype Control Cat. No. 566360; dashed line histogram or BD Horizon BV750 Mouse Anti-Human CD137 4-1BB antibody Cat. No. 569693; solid line histogram at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of CD137 4-1BB or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD137 4-1BB expression on stimulated Human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either BD Horizon BV750 Mouse IgG1, kappa Isotype Control Cat. No. 566360; dashed line histogram or BD Horizon BV750 Mouse Anti-Human CD137 4-1BB antibody Cat. No. 569693; solid line histogram at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of CD137 4-1BB or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD137 4-1BB expression on stimulated Human peripheral blood lymphocytes. Concanavalin A Con-A -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either BD Horizon BV750 Mouse IgG1, kappa Isotype Control Cat. No. 566360; dashed line histogram or BD Horizon BV750 Mouse Anti-Human CD137 4-1BB antibody Cat. No. 569693; solid line histogram at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of CD137 4-1BB or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative activated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.