

563735 ·
BDB Bioscience · Cat: 563735
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD11c expression on mouse dendritic cells. C57BL/6 mouse splenic leucocytes were cultured with recombinant mouse GM-CSF Cat. No. 554586; 5 ng/ml overnight. The cells were then harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV786 Hamster IgG1, lambda2 Isotype Control Cat. No. 563751; dashed line histogram or BD Horizon BV786 Hamster Anti-Mouse CD11c antibody Cat. No. 563735; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD11c expression on mouse dendritic cells. C57BL/6 mouse splenic leucocytes were cultured with recombinant mouse GM-CSF Cat. No. 554586; 5 ng/ml overnight. The cells were then harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV786 Hamster IgG1, lambda2 Isotype Control Cat. No. 563751; dashed line histogram or BD Horizon BV786 Hamster Anti-Mouse CD11c antibody Cat. No. 563735; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD11c expression on mouse dendritic cells. C57BL/6 mouse splenic leucocytes were cultured with recombinant mouse GM-CSF Cat. No. 554586; 5 ng/ml overnight. The cells were then harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV786 Hamster IgG1, lambda2 Isotype Control Cat. No. 563751; dashed line histogram or BD Horizon BV786 Hamster Anti-Mouse CD11c antibody Cat. No. 563735; solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.