BD HorizonTM BV786 Mouse Anti-Human IL-17F

Description

Multicolor flow cytometric analysis of IL-17F expression by resting and activated human peripheral blood CD4+ T cells. Human peripheral blood mononuclear cells were either unstimulated Upper Panels or stimulated Lower Panels with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139, 50 ng/ml plus Ionomycin Sigma I-0634, 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 5 hours. The cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with PerCP-Cy5.5 Mouse Anti-Human CD4 Cat. No. 560650 , BD Horizon BV786 Mouse Anti-Human IL-17F Cat. No. 564265 , PE Mouse Anti-Human IL-17A Cat. No. 560486/560487; Left Panels , and FITC Mouse Anti-Human IFN-gamma Cat. No. 554700/552887/561053; Right Panels antibodies. Two-color flow cytometric contour plots showing the correlated expression of IL-17F versus IL-17A or IFN- gamma were derived from CD4-gated events with the forward and side light scatter-characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of IL-17F expression by resting and activated human peripheral blood CD4+ T cells. Human peripheral blood mononuclear cells were either unstimulated Upper Panels or stimulated Lower Panels with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139, 50 ng/ml plus Ionomycin Sigma I-0634, 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 5 hours. The cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with PerCP-Cy5.5 Mouse Anti-Human CD4 Cat. No. 560650 , BD Horizon BV786 Mouse Anti-Human IL-17F Cat. No. 564265 , PE Mouse Anti-Human IL-17A Cat. No. 560486/560487; Left Panels , and FITC Mouse Anti-Human IFN-gamma Cat. No. 554700/552887/561053; Right Panels antibodies. Two-color flow cytometric contour plots showing the correlated expression of IL-17F versus IL-17A or IFN- gamma were derived from CD4-gated events with the forward and side light scatter-characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of IL-17F expression by resting and activated human peripheral blood CD4+ T cells. Human peripheral blood mononuclear cells were either unstimulated Upper Panels or stimulated Lower Panels with Phorbol 12-Myristate 13-Acetate PMA; Sigma P-8139, 50 ng/ml plus Ionomycin Sigma I-0634, 1 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 for 5 hours. The cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with PerCP-Cy5.5 Mouse Anti-Human CD4 Cat. No. 560650 , BD Horizon BV786 Mouse Anti-Human IL-17F Cat. No. 564265 , PE Mouse Anti-Human IL-17A Cat. No. 560486/560487; Left Panels , and FITC Mouse Anti-Human IFN-gamma Cat. No. 554700/552887/561053; Right Panels antibodies. Two-color flow cytometric contour plots showing the correlated expression of IL-17F versus IL-17A or IFN- gamma were derived from CD4-gated events with the forward and side light scatter-characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.