BD HorizonTM BV786 Mouse Anti-Human TIM-3 (CD366)

Description

Multiparameter flow cytometric analysis of TIM-3 CD366 expression on Human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 555516/561903; Lower Plots and with either BD Horizon BV786 Mouse IgG1, k Isotype Control Cat. No. 563330; Left Plots or BD Horizon BV786 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 568504; Right Plots at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Upper Plots - The bivariate pseudocolor density plots showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Lower Plots - The bivariate pseudocolor density plots showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of TIM-3 CD366 expression on Human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 555516/561903; Lower Plots and with either BD Horizon BV786 Mouse IgG1, k Isotype Control Cat. No. 563330; Left Plots or BD Horizon BV786 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 568504; Right Plots at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Upper Plots - The bivariate pseudocolor density plots showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Lower Plots - The bivariate pseudocolor density plots showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of TIM-3 CD366 expression on Human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 555516/561903; Lower Plots and with either BD Horizon BV786 Mouse IgG1, k Isotype Control Cat. No. 563330; Left Plots or BD Horizon BV786 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 568504; Right Plots at 0.5 mug/test. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Upper Plots - The bivariate pseudocolor density plots showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Lower Plots - The bivariate pseudocolor density plots showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.