BD HorizonTM BV786 Mouse Anti-Ki-67

Description

Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and noncycling human peripheral blood mononuclear cells. Proliferating cells from the human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line and noncycling peripheral blood mononuclear cells PBMC were fixed and permeabilized with 70 % ice cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BV786 Mouse Anti-Ki-67 antibody Cat. No. 563756 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cat. No. 555815/555816; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells Left Panel or PBMC Right Panel . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and noncycling human peripheral blood mononuclear cells. Proliferating cells from the human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line and noncycling peripheral blood mononuclear cells PBMC were fixed and permeabilized with 70 % ice cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BV786 Mouse Anti-Ki-67 antibody Cat. No. 563756 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cat. No. 555815/555816; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells Left Panel or PBMC Right Panel . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and noncycling human peripheral blood mononuclear cells. Proliferating cells from the human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line and noncycling peripheral blood mononuclear cells PBMC were fixed and permeabilized with 70 % ice cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BV786 Mouse Anti-Ki-67 antibody Cat. No. 563756 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cat. No. 555815/555816; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells Left Panel or PBMC Right Panel . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and noncycling human peripheral blood mononuclear cells. Proliferating cells from the human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line and noncycling peripheral blood mononuclear cells PBMC were fixed and permeabilized with 70 % ice cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BV786 Mouse Anti-Ki-67 antibody Cat. No. 563756 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cat. No. 555815/555816; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells Left Panel or PBMC Right Panel . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and noncycling human peripheral blood mononuclear cells. Proliferating cells from the human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line and noncycling peripheral blood mononuclear cells PBMC were fixed and permeabilized with 70 % ice cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BV786 Mouse Anti-Ki-67 antibody Cat. No. 563756 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cat. No. 555815/555816; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells Left Panel or PBMC Right Panel . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of Ki-67 expression by proliferating MOLT-4 and noncycling human peripheral blood mononuclear cells. Proliferating cells from the human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line and noncycling peripheral blood mononuclear cells PBMC were fixed and permeabilized with 70 % ice cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with BD Horizon BV786 Mouse Anti-Ki-67 antibody Cat. No. 563756 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then counterstained with BD Via-Probe Cat. No. 555815/555816; contains 7-Amino-Actinomycin D 7-AAD to stain DNA. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact MOLT-4 cells Left Panel or PBMC Right Panel . Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.