BD HorizonTM BV786 Mouse Anti-Mouse CD45.1

Description

Flow cytometric analysis of CD45.1 expression on Mouse splenic leukocytes. C57BL/6 Left Plot and SJL/J Right Plot Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV786 Mouse IgG2a, kappa Isotype Control Cat. No. 563732; dashed line histograms or BD Horizon BV786 Mouse Anti-Mouse CD45.1 antibody Cat. No. 569696; solid line histograms at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD45.1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45.1 expression on Mouse splenic leukocytes. C57BL/6 Left Plot and SJL/J Right Plot Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV786 Mouse IgG2a, kappa Isotype Control Cat. No. 563732; dashed line histograms or BD Horizon BV786 Mouse Anti-Mouse CD45.1 antibody Cat. No. 569696; solid line histograms at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD45.1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45.1 expression on Mouse splenic leukocytes. C57BL/6 Left Plot and SJL/J Right Plot Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV786 Mouse IgG2a, kappa Isotype Control Cat. No. 563732; dashed line histograms or BD Horizon BV786 Mouse Anti-Mouse CD45.1 antibody Cat. No. 569696; solid line histograms at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD45.1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45.1 expression on Mouse splenic leukocytes. C57BL/6 Left Plot and SJL/J Right Plot Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV786 Mouse IgG2a, kappa Isotype Control Cat. No. 563732; dashed line histograms or BD Horizon BV786 Mouse Anti-Mouse CD45.1 antibody Cat. No. 569696; solid line histograms at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD45.1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45.1 expression on Mouse splenic leukocytes. C57BL/6 Left Plot and SJL/J Right Plot Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV786 Mouse IgG2a, kappa Isotype Control Cat. No. 563732; dashed line histograms or BD Horizon BV786 Mouse Anti-Mouse CD45.1 antibody Cat. No. 569696; solid line histograms at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD45.1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45.1 expression on Mouse splenic leukocytes. C57BL/6 Left Plot and SJL/J Right Plot Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV786 Mouse IgG2a, kappa Isotype Control Cat. No. 563732; dashed line histograms or BD Horizon BV786 Mouse Anti-Mouse CD45.1 antibody Cat. No. 569696; solid line histograms at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing the expression of CD45.1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.