BD HorizonTM BV786 Mouse Anti-Mouse NK-1.1

Description

Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and with either BD Horizon BV786 Mouse IgG2a, kappa Isotype Control Cat. No. 565032, Left Plot or BD Horizon BV786 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 568224; Right Plot 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and with either BD Horizon BV786 Mouse IgG2a, kappa Isotype Control Cat. No. 565032, Left Plot or BD Horizon BV786 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 568224; Right Plot 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and with either BD Horizon BV786 Mouse IgG2a, kappa Isotype Control Cat. No. 565032, Left Plot or BD Horizon BV786 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 568224; Right Plot 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and with either BD Horizon BV786 Mouse IgG2a, kappa Isotype Control Cat. No. 565032, Left Plot or BD Horizon BV786 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 568224; Right Plot 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and with either BD Horizon BV786 Mouse IgG2a, kappa Isotype Control Cat. No. 565032, Left Plot or BD Horizon BV786 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 568224; Right Plot 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of NK-1.1 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 , then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and with either BD Horizon BV786 Mouse IgG2a, kappa Isotype Control Cat. No. 565032, Left Plot or BD Horizon BV786 Mouse Anti-Mouse NK-1.1 antibody Cat. No. 568224; Right Plot 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of NK1.1 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.