BD HorizonTM BV786 Rat Anti-Mouse CD62L

Description

Left Panels - Flow cytometric analysis of CD62L on mouse bone marrow cells. Mouse bone marrow cells were left untreated Left Panel or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Middle Left Panel . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV786 Rat IgG2a, kappa Isotype Control Cat. No. 563335, dashed line histogram or BD Horizon BV786 Rat Anti-Mouse CD62L antibody Cat. No. 564109, solid line histogram . Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Right Panels - Multicolor flow cytometric analysis of CD62L expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BV786 Rat IgG2a, kappa Isotype Control Middle Right Panel or BD Horizon BV786 Rat Anti-Mouse CD62L antibody Right Panel . Two-color flow cytometric contour plots showing the expression of CD62L or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Left Panels - Flow cytometric analysis of CD62L on mouse bone marrow cells. Mouse bone marrow cells were left untreated Left Panel or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Middle Left Panel . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV786 Rat IgG2a, kappa Isotype Control Cat. No. 563335, dashed line histogram or BD Horizon BV786 Rat Anti-Mouse CD62L antibody Cat. No. 564109, solid line histogram . Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Right Panels - Multicolor flow cytometric analysis of CD62L expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BV786 Rat IgG2a, kappa Isotype Control Middle Right Panel or BD Horizon BV786 Rat Anti-Mouse CD62L antibody Right Panel . Two-color flow cytometric contour plots showing the expression of CD62L or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Left Panels - Flow cytometric analysis of CD62L on mouse bone marrow cells. Mouse bone marrow cells were left untreated Left Panel or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Middle Left Panel . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon BV786 Rat IgG2a, kappa Isotype Control Cat. No. 563335, dashed line histogram or BD Horizon BV786 Rat Anti-Mouse CD62L antibody Cat. No. 564109, solid line histogram . Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Right Panels - Multicolor flow cytometric analysis of CD62L expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either BD Horizon BV786 Rat IgG2a, kappa Isotype Control Middle Right Panel or BD Horizon BV786 Rat Anti-Mouse CD62L antibody Right Panel . Two-color flow cytometric contour plots showing the expression of CD62L or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.