BD HorizonTM BV786 Rat Anti-Mouse IgM

Description

Two-color flow cytometric analysis of IgM expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE-CF594 Hamster Anti-Mouse CD3e Cat. No. 562286/562332 and either BV786 Rat IgG2a, kappa Isotype Control Cat. No. 563335; Left Panel or BV786 Rat Anti-Mouse IgM Cat. No. 564028, Right Panel . The fluorescence two-color dot plots were derived from gated events based on the light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD LSR II. Two-color flow cytometric analysis of IgM expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE-CF594 Hamster Anti-Mouse CD3e Cat. No. 562286/562332 and either BV786 Rat IgG2a, kappa Isotype Control Cat. No. 563335; Left Panel or BV786 Rat Anti-Mouse IgM Cat. No. 564028, Right Panel . The fluorescence two-color dot plots were derived from gated events based on the light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD LSR II. Two-color flow cytometric analysis of IgM expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE-CF594 Hamster Anti-Mouse CD3e Cat. No. 562286/562332 and either BV786 Rat IgG2a, kappa Isotype Control Cat. No. 563335; Left Panel or BV786 Rat Anti-Mouse IgM Cat. No. 564028, Right Panel . The fluorescence two-color dot plots were derived from gated events based on the light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD LSR II. Show More Two-color flow cytometric analysis of IgM expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE-CF594 Hamster Anti-Mouse CD3e Cat. No. 562286/562332 and either BV786 Rat IgG2a, kappa Isotype Control Cat. No. 563335; Left Panel or BV786 Rat Anti-Mouse IgM Cat. No. 564028, Right Panel . The fluorescence two-color dot plots were derived from gated events based on the light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD LSR II. Show More Two-color flow cytometric analysis of IgM expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE-CF594 Hamster Anti-Mouse CD3e Cat. No. 562286/562332 and either BV786 Rat IgG2a, kappa Isotype Control Cat. No. 563335; Left Panel or BV786 Rat Anti-Mouse IgM Cat. No. 564028, Right Panel . The fluorescence two-color dot plots were derived from gated events based on the light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD LSR II. Two-color flow cytometric analysis of IgM expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE-CF594 Hamster Anti-Mouse CD3e Cat. No. 562286/562332 and either BV786 Rat IgG2a, kappa Isotype Control Cat. No. 563335; Left Panel or BV786 Rat Anti-Mouse IgM Cat. No. 564028, Right Panel . The fluorescence two-color dot plots were derived from gated events based on the light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD LSR II.