BD HorizonTM BV786 Rat Anti-Mouse Ly-6C

Description

Multicolor flow cytometric analysis of Ly-6C expression on viable Mouse splenic leucocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and were either not further stained Autofluorescence; Left Plot or stained with BD Horizon BV786 Rat Anti-Mouse Ly-6C Cat. No. 569011/569012; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Autofluorescence versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Ly-6C expression on viable Mouse splenic leucocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and were either not further stained Autofluorescence; Left Plot or stained with BD Horizon BV786 Rat Anti-Mouse Ly-6C Cat. No. 569011/569012; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Autofluorescence versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Ly-6C expression on viable Mouse splenic leucocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and were either not further stained Autofluorescence; Left Plot or stained with BD Horizon BV786 Rat Anti-Mouse Ly-6C Cat. No. 569011/569012; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Autofluorescence versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Ly-6C expression on viable Mouse splenic leucocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and were either not further stained Autofluorescence; Left Plot or stained with BD Horizon BV786 Rat Anti-Mouse Ly-6C Cat. No. 569011/569012; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Autofluorescence versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Ly-6C expression on viable Mouse splenic leucocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and were either not further stained Autofluorescence; Left Plot or stained with BD Horizon BV786 Rat Anti-Mouse Ly-6C Cat. No. 569011/569012; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Autofluorescence versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Ly-6C expression on viable Mouse splenic leucocytes. BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD8a antibody Cat. No. 553035/561093 and were either not further stained Autofluorescence; Left Plot or stained with BD Horizon BV786 Rat Anti-Mouse Ly-6C Cat. No. 569011/569012; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Autofluorescence versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.