BD HorizonTM PE-CF594 Biosimilar Anti-Human TNF

Description

Multiparameter flow cytometric analysis of TNF Infliximab Biosimilar expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were either cultured without activators Unstimulated, Left Plot or were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration Stimulated, Right Plot in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The fixed and permeabilized cells were then stained in BD Perm/Wash Buffer with BD HorizonPE-CF594 Biosimilar Anti-Human TNF antibody Cat. No. 570955/571026 . The bivariate pseudocolor density plots showing the correlated expression of TNF versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and FlowJo Software. Multiparameter flow cytometric analysis of TNF Infliximab Biosimilar expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were either cultured without activators Unstimulated, Left Plot or were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration Stimulated, Right Plot in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The fixed and permeabilized cells were then stained in BD Perm/Wash Buffer with BD HorizonPE-CF594 Biosimilar Anti-Human TNF antibody Cat. No. 570955/571026 . The bivariate pseudocolor density plots showing the correlated expression of TNF versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and FlowJo Software. Multiparameter flow cytometric analysis of TNF Infliximab Biosimilar expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were either cultured without activators Unstimulated, Left Plot or were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration Stimulated, Right Plot in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The fixed and permeabilized cells were then stained in BD Perm/Wash Buffer with BD HorizonPE-CF594 Biosimilar Anti-Human TNF antibody Cat. No. 570955/571026 . The bivariate pseudocolor density plots showing the correlated expression of TNF versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and FlowJo Software. Show More Multiparameter flow cytometric analysis of TNF Infliximab Biosimilar expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were either cultured without activators Unstimulated, Left Plot or were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration Stimulated, Right Plot in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The fixed and permeabilized cells were then stained in BD Perm/Wash Buffer with BD HorizonPE-CF594 Biosimilar Anti-Human TNF antibody Cat. No. 570955/571026 . The bivariate pseudocolor density plots showing the correlated expression of TNF versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and FlowJo Software. Show More Multiparameter flow cytometric analysis of TNF Infliximab Biosimilar expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were either cultured without activators Unstimulated, Left Plot or were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration Stimulated, Right Plot in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The fixed and permeabilized cells were then stained in BD Perm/Wash Buffer with BD HorizonPE-CF594 Biosimilar Anti-Human TNF antibody Cat. No. 570955/571026 . The bivariate pseudocolor density plots showing the correlated expression of TNF versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and FlowJo Software. Multiparameter flow cytometric analysis of TNF Infliximab Biosimilar expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were either cultured without activators Unstimulated, Left Plot or were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration Stimulated, Right Plot in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The fixed and permeabilized cells were then stained in BD Perm/Wash Buffer with BD HorizonPE-CF594 Biosimilar Anti-Human TNF antibody Cat. No. 570955/571026 . The bivariate pseudocolor density plots showing the correlated expression of TNF versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and FlowJo Software.