BD HorizonTM PE-CF594 Mouse Anti-AhR

Description

Multiparameter flow cytometric analysis of AhR expression Top Plots - AhR expression in human peripheral blood mononuclear cells. PBMCs were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 and stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Plot or BD Horizon PE-CF594 Mouse Anti-AhR antibody Cat. No. 565790; Right Plot at 0.25 mug/test. Bottom Plots - AhR expression in mouse splenocytes. Mouse splenic leucocytes were similarly fixed, permeabilized, stained with antibodies at 0.5 mug/test, and analyzed. Two-parameter flow cytometric contour plots showing the correlated expression of AhR or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of AhR expression Top Plots - AhR expression in human peripheral blood mononuclear cells. PBMCs were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 and stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Plot or BD Horizon PE-CF594 Mouse Anti-AhR antibody Cat. No. 565790; Right Plot at 0.25 mug/test. Bottom Plots - AhR expression in mouse splenocytes. Mouse splenic leucocytes were similarly fixed, permeabilized, stained with antibodies at 0.5 mug/test, and analyzed. Two-parameter flow cytometric contour plots showing the correlated expression of AhR or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of AhR expression Top Plots - AhR expression in human peripheral blood mononuclear cells. PBMCs were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 and stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Plot or BD Horizon PE-CF594 Mouse Anti-AhR antibody Cat. No. 565790; Right Plot at 0.25 mug/test. Bottom Plots - AhR expression in mouse splenocytes. Mouse splenic leucocytes were similarly fixed, permeabilized, stained with antibodies at 0.5 mug/test, and analyzed. Two-parameter flow cytometric contour plots showing the correlated expression of AhR or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.