BD HorizonTM PE-CF594 Mouse Anti-Bcl-6

Description

Flow cytometric analysis of Bcl-6 expression in Ramos cells Left Panel . Human Ramos cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by intracellular staining with either BD Horizon PE-CF594 Mouse Anti-Human Bcl-6 antibody Cat. No. 562401, solid line histogram or a BD Horizon PE-CF594 mIgG1, kappa Isotype Control Cat. No. 562292; dashed line histogram . Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Bcl-6 expression in mouse B lymphocytes Middle and Right Panels . BALB/c mouse mesenteric lymph node cells were stained with APC Rat Anti-Mouse B220 Cat. No. 553092/561880 , PerCP-Cy5.5 Rat Anti-Mouse CD4 Cat. No. 550954/561115 , FITC Hamster Anti-Mouse Fas/CD95 Cat. No. 554257 , and BD Horizon V450 Rat Anti-Mouse IgD Cat. No. 560869 Abs. Cells were washed, resuspended in RPMI with 10 % FBS, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon PE-CF594 Mouse Anti-Bcl-6 antibody Cat. No. 562401 . A two-color flow cytometric dot plot shows the expression of IgD versus Fas/CD95 by B cells identified as CD4-B220+ from gated events with the forward and side light-scatter characteristics of intact lymphocytes Middle Panel . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Panel derived from gated cell subsets show intracellular Bcl-6 staining levels for mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of Bcl-6 expression in Ramos cells Left Panel . Human Ramos cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by intracellular staining with either BD Horizon PE-CF594 Mouse Anti-Human Bcl-6 antibody Cat. No. 562401, solid line histogram or a BD Horizon PE-CF594 mIgG1, kappa Isotype Control Cat. No. 562292; dashed line histogram . Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of Bcl-6 expression in mouse B lymphocytes Middle and Right Panels . BALB/c mouse mesenteric lymph node cells were stained with APC Rat Anti-Mouse B220 Cat. No. 553092/561880 , PerCP-Cy5.5 Rat Anti-Mouse CD4 Cat. No. 550954/561115 , FITC Hamster Anti-Mouse Fas/CD95 Cat. No. 554257 , and BD Horizon V450 Rat Anti-Mouse IgD Cat. No. 560869 Abs. Cells were washed, resuspended in RPMI with 10 % FBS, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon PE-CF594 Mouse Anti-Bcl-6 antibody Cat. No. 562401 . A two-color flow cytometric dot plot shows the expression of IgD versus Fas/CD95 by B cells identified as CD4-B220+ from gated events with the forward and side light-scatter characteristics of intact lymphocytes Middle Panel . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Panel derived from gated cell subsets show intracellular Bcl-6 staining levels for mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of Bcl-6 expression in Ramos cells Left Panel . Human Ramos cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by intracellular staining with either BD Horizon PE-CF594 Mouse Anti-Human Bcl-6 antibody Cat. No. 562401, solid line histogram or a BD Horizon PE-CF594 mIgG1, kappa Isotype Control Cat. No. 562292; dashed line histogram . Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Bcl-6 expression in mouse B lymphocytes Middle and Right Panels . BALB/c mouse mesenteric lymph node cells were stained with APC Rat Anti-Mouse B220 Cat. No. 553092/561880 , PerCP-Cy5.5 Rat Anti-Mouse CD4 Cat. No. 550954/561115 , FITC Hamster Anti-Mouse Fas/CD95 Cat. No. 554257 , and BD Horizon V450 Rat Anti-Mouse IgD Cat. No. 560869 Abs. Cells were washed, resuspended in RPMI with 10 % FBS, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon PE-CF594 Mouse Anti-Bcl-6 antibody Cat. No. 562401 . A two-color flow cytometric dot plot shows the expression of IgD versus Fas/CD95 by B cells identified as CD4-B220+ from gated events with the forward and side light-scatter characteristics of intact lymphocytes Middle Panel . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Panel derived from gated cell subsets show intracellular Bcl-6 staining levels for mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry was performed using a BD LSR II Flow Cytometer System.