BD HorizonTM PE-CF594 Mouse Anti-Human Aiolos

Description

Multicolor flow cytometric analysis of Aiolos expression in human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and FITC Mouse Anti-Human CD3 antibody Cat. No. 555916 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Plots or BD Horizon PE-CF594 Mouse Anti-Human Aiolos antibody Cat. No. 567871/567872; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Upper Plots: Pseudocolor bivariate density plots showing the correlated expression of Aiolos or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side-light scattering characteristics of intact lymphocytes. Bottom Plots: Pseudocolor bivariate density plots showing the correlated expression of Aiolos or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side-light scattering characteristics of intact lymphocytes. Multicolor flow cytometric analysis of Aiolos expression in human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and FITC Mouse Anti-Human CD3 antibody Cat. No. 555916 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Plots or BD Horizon PE-CF594 Mouse Anti-Human Aiolos antibody Cat. No. 567871/567872; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Upper Plots: Pseudocolor bivariate density plots showing the correlated expression of Aiolos or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side-light scattering characteristics of intact lymphocytes. Bottom Plots: Pseudocolor bivariate density plots showing the correlated expression of Aiolos or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side-light scattering characteristics of intact lymphocytes. Show More Multicolor flow cytometric analysis of Aiolos expression in human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and FITC Mouse Anti-Human CD3 antibody Cat. No. 555916 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Plots or BD Horizon PE-CF594 Mouse Anti-Human Aiolos antibody Cat. No. 567871/567872; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Upper Plots: Pseudocolor bivariate density plots showing the correlated expression of Aiolos or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side-light scattering characteristics of intact lymphocytes. Bottom Plots: Pseudocolor bivariate density plots showing the correlated expression of Aiolos or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side-light scattering characteristics of intact lymphocytes.