BD HorizonTM PE-CF594 Mouse Anti-Human CD194

Description

Multicolor flow cytometric analysis of CD194 CCR4 expression on human peripheral blood lymphocytes. Human whole blood was treated with BD PharmLyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842; Top Panels and Alexa Fluor 647 Mouse Anti-Human CD8a Cat. No. 557708; Bottom Panels antibodies, and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panels or BD Horizon PE-CF594 Mouse Anti-Human CD194 CCR4 antibody Cat. No. 565391; Right Panels . Two-color flow cytometric contour plots showing the correlated expression patterns of CD194 CCR4 or Ig Isotype control staining versus CD4 or CD8a were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometr9c analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD194 CCR4 expression on human peripheral blood lymphocytes. Human whole blood was treated with BD PharmLyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842; Top Panels and Alexa Fluor 647 Mouse Anti-Human CD8a Cat. No. 557708; Bottom Panels antibodies, and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panels or BD Horizon PE-CF594 Mouse Anti-Human CD194 CCR4 antibody Cat. No. 565391; Right Panels . Two-color flow cytometric contour plots showing the correlated expression patterns of CD194 CCR4 or Ig Isotype control staining versus CD4 or CD8a were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometr9c analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD194 CCR4 expression on human peripheral blood lymphocytes. Human whole blood was treated with BD PharmLyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were then stained with FITC Mouse Anti-Human CD4 Cat. No. 555346/561005/561842; Top Panels and Alexa Fluor 647 Mouse Anti-Human CD8a Cat. No. 557708; Bottom Panels antibodies, and either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; Left Panels or BD Horizon PE-CF594 Mouse Anti-Human CD194 CCR4 antibody Cat. No. 565391; Right Panels . Two-color flow cytometric contour plots showing the correlated expression patterns of CD194 CCR4 or Ig Isotype control staining versus CD4 or CD8a were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometr9c analysis was performed using a BD LSR II Flow Cytometer System.