
567140 · 100 Tests
BDB Bioscience · Cat: 567140
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Description
Flow cytometric analysis of CD278 expression on stimulated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; dashed line histogram or BD Horizon PE-CF594 Mouse Anti-Human CD278 antibody Cat. No. 567140/567141; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD278 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD278 expression on stimulated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; dashed line histogram or BD Horizon PE-CF594 Mouse Anti-Human CD278 antibody Cat. No. 567140/567141; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD278 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD278 expression on stimulated Human peripheral blood lymphocytes. Phytohemagglutinin PHA -stimulated 3 days peripheral blood mononuclear cells PBMC were stained with either BD Horizon PE-CF594 Mouse IgG1, kappa Isotype Control Cat. No. 562292; dashed line histogram or BD Horizon PE-CF594 Mouse Anti-Human CD278 antibody Cat. No. 567140/567141; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD278 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.